Combining the power of HPLC and GC

Both GC and LC are widely used in today’s analytical lab. Each technique has its advantages. In recent years
HPLC has increased in range of application mostly because shorter sample preparation.

Yet GC still offers the best sensitivity and separation power.
The coupling of LC to GC offers the best of both world:

  • Simple sample preparation
  • Sample enrichment
  • High resolution of compounds
  • High sensitivity

In LC-GC technique, the sample is separated using typically NPLC (Normal Phase Liquid Chromatography).
The LC allows selective isolation of groups of compounds then transfer to the GC by mean of on-column large volume injection.

Following a long tradition of chromatography innovation and experience,
Brechbühler AG now commercializes Thermo’s LC-GC System.
Brechbühler brings exciting enhancement to the technique as well as its knowledge and excellent customer support.



Q Exactive™ GC hybrid quadrupole Orbitrap mass spectrometer coupled with on-line LC-GC 9000 System


The principle of the LC-GC coupling is simple


After separation on the LC column the fraction of interest is transferred via the transfer valve onto the GC column by large volume injection technique. The fraction transferred is analyzed by the GC or GC/MS. A backflush of the LC column can be performed at any time. Because of large volume injection the sensitivity is greatly improved

Automation at your fingertip

The dedicated software makes the LC-GC 9000 a routine instrument. Once the method is setup, a sequence of sample can be programmed. The software controls all the instruments (Phoenix pump, Trace GC, PAL Autosampler and Exachrom acquisition system).

Mode of operation

Standard mode:
one transfer per LC run


Multitransfer mode:
Several transfer per LC Run. The pumps are placed on Hold after each transfer for the duration of the GC run. This mode can be used for LCxGC experiments


Peak detection transfer:
The transfer is triggered by the detection of a peak on the UV detector.

Dual channel transfer

The LC-GC 9000 system is now capable of dual channel transfer on a same LC run.The Switching (or auxiliary) valve is configured as a second transfer valve for the 2nd channel. 


The Trace 13XX GC already equipped with the auxiliary carrier and valve and temperature control module is fitted with a second Solvent Vapor Exit (SVE) valve.


The LC-GC Software has been modified to include an additional parameter for the amount of the MOAH internal standard amount. This amount is sent to Chromeleon for calculation. Reports have been modified to include both MOSH and MOAH data.

For complex matrices

LC-GC coupling has been successfully used in the following fields:


  • Food samples
  • Flavor & Fragrance samples
  • Petrochemical and industrial samples
  • Environmental samples
  • Pharmaceutical samples
  • Biological samples.


Any sample in complex matrices.


There are many applications published using LC-GC techniques.
For more information on the technique please refer to Dr.. K. Grob & Maurus Biedermann work.
Another good starting point is a paper by Luigi Mondelo: On-line Coupled LC–GC: Theory and Applications

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