Chiral separation and purification have become increasingly important in pharmacology, food science, agrochemistry and other areas
Chiral Supercritical Fluid Chromatography (SFC) with CO2 potentially offers a shorter analysis time due to higher diffusivity and lower viscosity and reduction of the amount of organic solvents
Using the G-factor, a circular dichroism (CD) detector can effectively be used for determination of chiral purity of chromatographic peaks. G-factor is defined as CD/UV or ΔAU/AU and is independent of the chromatographic peak concentration
The Jasco CD-2095 Detector measures optical isomers with circular dichromatic absorption between 220 and 420 nm with high sensitivity and excellent selectivity. Compared to an optical rotation detector, it is generally 10 to 100 times more sensitive.
It can measure both CD and UV chromatograms as well as g-factor (CD/UV) chromatograms. Since g-factor in particular has a proportional relationship with the compositional ratio of optical isomer samples, the CD-2095 can perform compositional measurements and high purity fractionation for non-separated peaks by, for example, measuring the chromatogram of the g-factor. In addition, spectral measurement for CD and UV is possible by stopped-flow spectrometry.