Aeris™ - Core-Shell HPLC/UHPLC Technology for Proteins and Peptides

Aeris™ – core-shell HPLC/UHPLC columns specially designed for the analysis of proteins and peptides.  These new high-performance columns deliver higher resolution and peak capacities than other bioseparation media, producing sub-2 micron results on any LC instrument.  With virtually no bleed, Aeris columns are highly compatible with MS.

 

Aeris columns are offered in wide- and small-pore configurations. Aeris WIDEPORE 3.6-micron core-shell columns are optimized for the separation of intact proteins and polypeptides, and are available in three selectivities – XB-C18, XB-C8 and C4. Because the 3.6-micron core-shell particles produce lower backpressures than traditional media, longer or coupled columns can be used to increase resolving power on both HPLC and UHPLC instruments.

Aeris PEPTIDE 3.6-micron and 1.7-micron columns, designed with a small pore size, are recommended for the separation of low molecular weight peptides and for peptide mapping. The novel XB-C18 stationary phase provides the ideal surface chemistry for resolving peptides. Aeris PEPTIDE 3.6-micron particles offer ultra-high performance at backpressures compatible with both HPLC and UHPLC instruments. On UHPLC systems, the 1.7-micron Aeris particles can deliver higher peak capacities than fully porous sub-2 micron columns.

“The new Aeris columns are ‘plug and play,’ requiring very little work in method transfer,” comments Kari Kelly, brand manager for Phenomenex. “With a little method optimization, users can achieve even higher resolution and better performance, and our PhenoLogix support team is ready to help customers with their switch to Aeris.”

 

Introducing Aeris core-shell HPLC

UHPLC columns for proteins and peptides. A new family of core-shell particles specifically designed to bring ultra-high resolution and peak capacity to bioseparations on ANY system.

 

Aeris™ PEPTIDE Columns for Peptide and Peptide Mapping Separations

Based on core-shell particle technology, Aeris PEPTIDE particles are designed with small pores, inert XB-C18 surface chemistry, and two different particle sizes (3.6 µm and 1.7 µm) to meet the resolution demands of chromatographers performing complex peptide and peptide map separations on HPLC and/or UHPLC systems.

 

Aeris™ PEPTIDE columns are built for the following:

  • Synthetic peptide impurity analysis
  • Peptide mapping
  • Identifying protein modifications
    Glycosylation
    Substitution
    Truncation
  • Analyzing post-translational modifications
    Deamidation
    Oxidation
    Deletions

Aeris™ WIDEPORE Columns for Intact Protein and Polypeptide Separations

Aeris WIDEPORE columns are packed with 3.6 µm core-shell particles that are specially engineered with a thin porous shell, large pores, and sterically protected XB surface chemistry to address the inherent separation challenges of proteins and peptides. This unique mix of features results in low backpressures, fast rates of diffusion, and excellent selectivity, generating exceptional chromatographic resolution on both HPLC and UHPLC systems.

 

Recommended for…

  • Protein structural characterization
  • Stability indicating assays
  • Post-translational modification identification
  • PEGylated proteins, antibodies, biogenerics, etc.
  • Impurity profiling
  • Peptide mapping
     

Aeris™ Coreshell Technology

Core-shell particle technology provides striking increases in peak capacity and resolution at lower backpressure, giving chromatographers the ability to achieve ultra-high performance on ANY system, HPLC or UHPLC.

A uniform porous silica layer is grown around a solid, spherical silica core, providing effective retention and selectivity with improved resolution, speed, and recovery. Next, optimizing the pore size and shell thickness for intact proteins or smaller peptide fragments provides well-defined depth penetration of biomolecules leading to maximum separation power.

 

TEM and SEM of Aeris™ PEPTIDE 3.6 µm Core-Shell Particles

The precise architecture of core-shell particles provides dramatic leaps in performance in two important ways:

Step 1

The thin, porous layer, or "shell", decreases the diffusion path length, thus reducing the time it takes for biomolecules to adsorb/desorb into and out of the particles.

Step 2

Uniform sizing and shape of the particles along with tight packing specifications reduces losses in efficiency and performance due to band broadening.