Brechbühler AG: Analytical SFC

Analytical Supercritical Fluid Chromatography

The physical characteristics exhibited by supercritical fluid include a diffusion coefficient that is a hundred times greater than liquid and a viscosity that is at least one figure smaller. A supercritical fluid chromatography system, such as a mobile phase medium, can rapidly perform separations without any drop in separation efficiency, even at fast flow rates, due to a rapid mass transfer inside the column when compared with high-speed liquid chromatography. In addition, when carbon dioxide is used as the medium, gasification will occur keeping the separated and fractionated sample at a constant temperature, making this technique capable of highly efficient refining with few post-processing hassles, such as the elimination of solvents after preparative isolation. This offers a host of advantages, including cost cuts related to the expense of purchasing and discarding organic solvents, high throughput analysis, and rapid preparative isolation over a short period of time.
As a gradient elution technique, SFC is capable of varying the three parameters of pressure, temperature, and modifier solvent volume when separating oligomers and constituents with differing characteristics.